Isolation of Markers from Recently Transposed LINE-1 Retrotransposons

Isolation of Markers from Recently Transposed LINE-1 Retrotransposons

Blog Article

Many previous techniques for the isolation of endogenous retroelements such as LINE-1 retrotransposons have produced major sampling bias or required laborious procedures.These problems led to the isolation of only older elements in some cases.In other cases, sequal eclipse 5 battery specialized systems were required for the isolation of recently transposed elements.

We report here a system for the easy isolation of markers from a wide range of LINE-1 elements and the screening of recently transposed elements from that population.This is accomplished by the use of PCR with degenerate primers specific for conserved regions of the reverse transcriptase gene, a modified screening vector, and a refined blue/white colony assay that screens for amplified DNA containing open reading frames.This read more method should be applicable to searches for endogenous retroviruses.